Description
Product Overview
The EpiQuik Global Di-Methyl H3K9 Quantification Kit is a colorimetric assay designed to quantify the level of di‑methylation at histone H3 lysine 9 (H3K9) in a microplate format. Developed by Epigentek, a recognized leader in epigenetics research tools, the kit provides a streamlined workflow that converts histone extracts into a measurable color change, enabling researchers to assess epigenetic modifications with high precision. Each kit contains 48 individual assay wells, allowing simultaneous analysis of multiple samples or experimental conditions. The reagents are supplied in a ready‑to‑use format, eliminating the need for complex reagent preparation and reducing variability between runs. The assay is compatible with standard 96‑well plates, spectrophotometric plate readers, and can be integrated into existing laboratory automation pipelines.
Usage
This kit is intended for use in academic, biotech, and pharmaceutical laboratories focused on chromatin biology, epigenetics, and gene regulation studies. It is particularly valuable for researchers investigating the role of H3K9 methylation in transcriptional silencing, heterochromatin formation, and disease‑related epigenetic alterations. The assay works with histone extracts prepared from cultured cells, tissue samples, or purified nucleosome preparations. Users add their samples to the assay wells, followed by the kit’s proprietary detection reagents. After an incubation period, the development of a blue‑purple color is measured at 450 nm, providing a quantitative readout that correlates directly with the amount of H3K9 di‑methylation present. The protocol requires minimal hands‑on time—approximately 30 minutes for sample preparation and 1 hour for incubation—making it suitable for high‑throughput screening projects.
Why Choose Us
Choosing the EpiQuik H3K9 Quantification Kit means selecting a product backed by rigorous quality control and extensive validation data. Epigentek has performed cross‑lab reproducibility studies that demonstrate a coefficient of variation below 10 % across a wide dynamic range. The kit’s colorimetric detection system avoids the need for specialized fluorescence equipment, reducing capital costs while maintaining sensitivity comparable to antibody‑based ELISA methods. Each batch is accompanied by a detailed certificate of analysis, and the company offers technical support staffed by scientists experienced in histone modification assays. In addition, the kit is supported by a library of peer‑reviewed publications that cite its use in diverse research contexts, providing confidence that the assay meets the standards of top‑tier journals.
Key Features
- High‑throughput 48‑well format enables parallel processing of many samples.
- Colorimetric readout compatible with standard plate readers eliminates need for expensive equipment.
- Validated sensitivity detects low‑level H3K9 di‑methylation, supporting subtle epigenetic studies.
- Simple, two‑step protocol reduces hands‑on time and minimizes user error.
- Dedicated technical support and detailed protocol guide ensure successful implementation.
FAQ
What type of samples can I use with this kit?
The assay accepts histone extracts prepared from cultured cells, primary tissue, or purified nucleosome preparations. Samples should be quantified for protein concentration before loading to ensure accurate comparison across wells.
Is the kit compatible with automated liquid handling systems?
Yes, the reagents are supplied in a format that works well with most 96‑well liquid handling platforms. The protocol can be adapted for robotic dispensing without loss of assay performance.
How does the sensitivity of this colorimetric assay compare to ELISA?
Our validation data show comparable sensitivity to traditional ELISA kits, with a detection limit in the low nanogram range. The colorimetric approach provides a broader dynamic range and reduces background signal.
Can I multiplex this assay with other histone modification kits?
The kit is designed for single‑target analysis, but you can run separate assays for different modifications on the same plate, provided you follow the recommended washing steps to avoid cross‑reactivity.
What support is available if I encounter technical issues?
Epigentek offers email and phone support staffed by scientists familiar with histone assays. Users also have access to an online knowledge base, protocol videos, and troubleshooting guides.
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