Bradford Protein Assay with BSA Standard 500 Assays Cepham Sciences

Description

Product Overview

The Bradford Protein Assay with BSA Standard is a highly regarded colorimetric assay kit designed for accurate protein quantification in a variety of laboratory settings. Each kit contains 500 ready‑to‑use assay reagents, calibrated against a bovine serum albumin (BSA) protein standard, ensuring consistent and reproducible results across multiple experiments. The assay utilizes Coomassie Brilliant Blue G‑250 dye, which binds to protein molecules and produces a measurable shift in absorbance that correlates directly with protein concentration. This principle provides a rapid, sensitive, and cost‑effective method for determining total protein content in samples ranging from cell lysates to purified protein solutions.

Manufactured by Cepham Sciences, a trusted name in life‑science supplies, the kit adheres to strict quality control standards and is packaged to maintain reagent stability over extended storage periods. The product is suitable for both cuvette‑based spectrophotometric measurements and microplate formats, offering flexibility for low‑throughput or high‑throughput workflows. Detailed instructions accompany each kit, guiding users through sample preparation, reagent mixing, incubation times, and data analysis, thereby minimizing variability and user error.

Usage

This assay is ideal for researchers performing Western blot normalization, enzyme‑linked immunosorbent assays (ELISA), and routine protein quantification in biochemistry, molecular biology, and cell biology laboratories. The straightforward protocol allows users to measure protein concentrations within minutes, making it an excellent choice for time‑sensitive projects such as kinetic studies, dose‑response experiments, and quality control of protein production batches. The kit’s compatibility with standard laboratory equipment, including UV‑Vis spectrophotometers and plate readers, ensures seamless integration into existing workflows without the need for specialized instrumentation.

Because the assay is based on a simple color change, it can be performed in environments ranging from academic teaching labs to industrial research facilities. The robust nature of the reagents tolerates a wide pH range and common buffer components, reducing the need for extensive sample cleanup. Users can confidently apply the assay to diverse sample types, including clarified lysates, purified recombinant proteins, and complex biological fluids, while maintaining high accuracy and low background interference.

Why Choose Us

Cepham Sciences has built a reputation for delivering reliable, high‑quality assay kits that meet the rigorous demands of modern research. Our Bradford Protein Assay is manufactured under GMP‑like conditions, with each batch undergoing thorough validation to confirm linearity, sensitivity, and reproducibility. The inclusion of a BSA protein standard in every kit eliminates the need for external calibration, saving time and reducing potential sources of error.

In addition to product quality, Cepham Sciences provides comprehensive technical support, including detailed protocol PDFs, troubleshooting guides, and direct access to experienced scientists who can assist with experimental design and data interpretation. Our commitment to customer satisfaction extends to fast shipping, transparent pricing, and a clear return policy for any defective components.

Choosing our Bradford Protein Assay means investing in a solution that combines scientific rigor with user‑friendly design, ensuring that your research data is both accurate and reproducible. The kit’s long shelf life, stable reagents, and consistent performance across multiple assays make it a cost‑effective choice for laboratories that require dependable protein measurement tools on a regular basis.

Key Features

• Provides 500 ready‑to‑use assays, reducing preparation time and ensuring batch consistency.
• Utilizes Coomassie G‑250 dye for rapid, sensitive detection of protein concentrations as low as 1 µg/mL.
• Compatible with both cuvette spectrophotometers and 96‑well microplate readers for flexible workflow options.
• Includes a calibrated BSA protein standard, eliminating the need for external standards and simplifying data analysis.
• Backed by Cepham Sciences technical support and a satisfaction guarantee for reliable after‑sales service.

FAQ

What is the detection range of the Bradford Protein Assay?

The assay reliably detects protein concentrations from 1 µg/mL up to 2000 µg/mL, providing a broad linear range suitable for most laboratory applications.

Can the assay be used with samples containing detergents or reducing agents?

Yes, the Bradford assay tolerates low concentrations of common detergents such as Triton X‑100 and SDS, as well as reducing agents like DTT, though extreme concentrations may affect dye binding and should be evaluated during method optimization.

Is the kit suitable for high‑throughput screening?

Absolutely. The kit’s microplate format allows simultaneous processing of up to 96 samples, and the rapid color development (typically 5–10 minutes) makes it ideal for high‑throughput screening projects.

How should the reagents be stored?

All reagents should be stored at 2–8 °C, protected from light. The dye concentrate remains stable for up to 12 months when kept under these conditions.

What support does Cepham Sciences offer if I encounter problems?

Cepham Sciences provides a dedicated technical support line, detailed troubleshooting documentation, and email assistance from experienced scientists to help resolve any assay‑related issues quickly.