Bicinchoninic Acid BCA Protein Assay Kit Cepham Sciences 500 Assays

Description

Product Overview

The Bicinchoninic Acid (BCA) Protein Assay Kit from Cepham Sciences provides a robust, colorimetric method for quantifying total protein in a wide variety of sample types. The assay relies on the reduction of Cu²⁺ to Cu¹⁺ by protein molecules under alkaline conditions, followed by the formation of a purple‑blue complex with bicinchoninic acid that absorbs at 562 nm. This chemistry delivers high sensitivity, a linear range from 20 µg mL⁻¹ to 2 mg mL⁻¹, and excellent reproducibility across multiple runs.

The kit is supplied with all necessary reagents, including BSA standard (2 mg mL⁻¹), Reagent A, Reagent B, and a detailed standard operating procedure that guarantees consistent performance. All components are packaged in calibrated containers to ensure accurate pipetting and minimal waste. The reagents are stable at 2‑8 °C for up to 12 months, providing long‑term reliability for busy laboratories.

In addition to the core chemistry, the formulation has been optimized to reduce protein‑to‑protein variation, offering more precise results than traditional dye‑binding methods such as Bradford. The assay is compatible with a broad pH range (7.0‑9.0) and tolerates up to 5 % (v/v) of common detergents, making it suitable for complex biological matrices. The kit’s documentation includes troubleshooting tips, data analysis guidelines, and recommended storage conditions.

Usage

The assay is designed for laboratory environments where speed, accuracy, and compatibility with common detergents are essential. Researchers can apply the kit to cell lysates, tissue extracts, purified proteins, and even complex mixtures that contain SDS, Triton X‑100, or NP‑40 without compromising signal intensity. The protocol requires only two reagent additions and a short incubation at 37 °C for 30 minutes, making it suitable for high‑throughput workflows in proteomics, enzyme kinetics, and drug discovery projects.

Sample preparation is straightforward: dilute the unknown sample in the provided assay buffer, add Reagent A, then Reagent B, and incubate. After the incubation period, the absorbance is measured at 562 nm using a standard plate reader. The resulting absorbance values are compared against the BSA standard curve to calculate protein concentration. The kit supports both 96‑well and 384‑well plate formats, enabling flexibility for different throughput requirements.

Because the assay tolerates up to 5 % detergent, users can directly assay crude lysates without extensive cleanup, saving time and reducing sample loss. The method also exhibits low background interference from reducing agents such as DTT and β‑mercaptoethanol, further expanding its applicability. The 500‑assay capacity enables large‑scale screening campaigns while maintaining low per‑sample cost.

Why Choose Us

Cepham Sciences has a long history of delivering reliable life‑science reagents backed by rigorous quality control. Each batch of the BCA Protein Assay Kit undergoes independent verification of reagent concentration, purity, and performance against the manufacturer’s standard operating procedure. The product is covered by a money‑back guarantee if it does not meet the documented specifications, reflecting confidence in its consistency.

Our technical support team provides personalized assistance for method optimization, troubleshooting, and data interpretation, ensuring that users achieve accurate results from the first experiment. We also offer a comprehensive online resource library that includes protocol videos, validation data sheets, and application notes covering a range of sample types and experimental designs.

Environmental responsibility is a core value; all packaging materials are recyclable, and the reagents are formulated without hazardous solvents. By choosing Cepham Sciences, laboratories benefit from a trusted partner that prioritizes product quality, customer service, and sustainable practices.

Key Features

• High sensitivity colorimetric detection at 562 nm for reliable protein quantification
• Detergent‑compatible formulation works with SDS, Triton X‑100, and NP‑40
• Includes BSA standard and reagents for up to 500 micro‑assays
• Simple two‑step protocol reduces hands‑on time and minimizes error
• Comprehensive technical support and money‑back performance guarantee

FAQ

What is the detection range of the BCA assay?

The assay provides a linear response for protein concentrations between 20 µg mL⁻¹ and 2 mg mL⁻¹, covering most routine laboratory applications.

Can the assay be used with samples containing detergents?

Yes, the formulation is specifically designed to tolerate common detergents such as SDS, Triton X‑100, and NP‑40 without reducing assay sensitivity.

How many assays can be performed with one kit?

The kit contains enough reagents for 500 individual micro‑assays, allowing extensive experimental series or high‑throughput screening.

Is there a guarantee for assay performance?

Cepham Sciences offers a money‑back guarantee if the kit does not meet the performance specifications outlined in the supplied SOP.

What support is available for troubleshooting?

Our technical support team is available via email and phone to help optimize protocols, interpret results, and resolve any issues that arise during use.